• Users Online: 286
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2012  |  Volume : 37  |  Issue : 3  |  Page : 172-177

Detection of tyrosine hydroxylase m-RNA in the peripheral blood of neuroblastoma patients and its relation to treatment response


1 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
2 Department of Pediatric Oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
3 Department of Clinical Pathology, Faculty of Medicine, South Valley University, Qena, Egypt

Correspondence Address:
Hosny B. Hamed
Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, 7111 Assiut
Egypt
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.7123/01.EJH.0000416548.07291.ab

Rights and Permissions

Background

Neuroblastoma (NB) is the most common malignant solid tumor in childhood and, among all childhood malignancies, is second only to leukemia. NB originates before birth in the neural crest. Tyrosine hydroxylase (TH) is the first enzyme in the pathway of catecholamine synthesis and the detection of TH m-RNA is very useful as a tumor marker for NB.

Aim of the study

The aim of the present study is to detect the TH m-RNA in the peripheral blood of patients with NB by real-time reverse transcriptase-PCR (RT-PCR) at diagnosis and during therapy to determine the role of TH m-RNA in the diagnosis of NB and its relation to the treatment response.

Patients and methods

Blood samples were collected from 32 children with advanced stages NB (stages III and IV), either newly diagnosed (n=11) or receiving treatment (n=21). Another 12 blood samples were also obtained from age-matched children with other pediatric cancers as a control group. We used real-time RT-PCR for the detection of TH m-RNA.

Results

TH m-RNA expression was detected in peripheral blood samples obtained from five of 11 (45.4%) newly diagnosed cases and in five of 19 (26.3%) children receiving treatment. A significant difference was found between stages (III and VI) at presentation (P=0.04), LDH level (P=0.04), and bone marrow disease (P=0.025) with the detection of TH m-RNA in peripheral blood. Although no significant effect on survival outcomes had been reported in our patients, there were significant differences (P=0.04) in the response to treatment and TH m-RNA expression.

Conclusion

Detection of TH m-RNA by real-time RT-PCR is a reliable, quick, and easy way to detect the expression of NB cell in blood at diagnosis. Also, TH expression can be used as a tumor marker for the accurate diagnosis of NB and a predictor of treatment response.



[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1055    
    Printed29    
    Emailed0    
    PDF Downloaded89    
    Comments [Add]    

Recommend this journal