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ORIGINAL ARTICLE
Year : 2019  |  Volume : 44  |  Issue : 2  |  Page : 124-127

Study of m65 hepatocyte death marker in multitransfused patients with β-thalassemia major in upper Egypt


1 Department of Clinical Pathology, Faculty of Medicine, Aswan University, Aswan, Egypt
2 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt

Correspondence Address:
HebatAllah Abdellatif
Department of Clinical Pathology, Faculty of Medicine, Aswan University, Aswan 81528
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1110-1067.271082

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Introduction Iron overload is a common complication in patients with β-thalassemia major (β-TM) and is defined as serum ferritin level higher than 1000 ng/ml. Deposition of iron in body organs, mainly in the liver, leads to hepatocytes damage. Keratins are the major epithelial-specific subgroup of intermediate filament proteins. Increased apoptosis and/or necrosis play a role in the pathogenesis and determination of disease progression. Keratin 18 is cleaved by caspases during apoptosis and creates M30 fragments. Soluble intact K18 (M65) is also released from cells during cell death. Patients and methods A prospective case–control study of 70 multitransfused patients with β-TM and 22 controls was conducted in Paediatric Haematology Unit of Assiut University hospital from January 2016 till April 2017. The study participants were screened for liver enzymes, hepatitis markers (hepatitis C virus antibodies and hepatitis B virus antigen), and serum ferritin levels. Circulating levels of human keratin 18-M65 (K18-M65) were measured by enzyme-linked immunosorbent assay. Results Serum M65 levels were higher in patients’ group compared with healthy controls (P<0.001). M65 serum levels were also positively correlated with the serum levels of liver transaminases including aspartate aminotransferase (AST) (δ-0.470, P<0.001) and alkaline phosphatase (δ=0.180, P=0.043), and serum ferritin (δ=0.450, P=0.043). Conclusion M65 serum levels increased significantly with increased levels of serum ferritin and liver enzymes (aspartate aminotransferase and alkaline phosphatase) between β-TM patient groups.


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