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ORIGINAL ARTICLE
Year : 2019  |  Volume : 44  |  Issue : 4  |  Page : 254-259

Coexistence of (CCND1/PRAD-1/BCL1) juxtaposition and drug resistance gene is a marker for vincristine-resistant B-non-Hodgkin’s lymphoma


1 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Asyut, Egypt
2 Assitant Professor of clinical pathology, Assiut University, Egypt

Correspondence Address:
Sahar El Gammal
Clinical Pathology Department, Faculty of Medicine, Assiut University, Asyut
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ejh.ejh_27_19

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Background CCND1/PRAD-1/BCL1 juxtaposition activates immunoglobulin heavy chain (IgH) enhancing effect on cyclin D1 expression. This leads to overexpression of cyclin D1, a cell-cycle regulator. Cyclin D1 can function as an oncogene growth advantage for tumor cells by way of cell-cycle progression. Multidrug resistance (MDR)1 gene expression leads to overproduction of P-glycoprotein (P-gp) which leads to chemotherapy resistance causing poor prognosis and short survival [overall survival (OS) and disease free survival(DFS)] in B-non-Hodgkin’s lymphoma (NHL). Objective To evaluate BCL1/IgH juxtaposition by fluorescent in-situ hybridization (FISH) and MDR1 protein expression by immunohistochemistry in B-non Hodgkin lymphoma (B-NHL) and to clarify if these genes have some relevance to developing vincristine treatment resistance of B-NHL and their correlation with clinical outcome. Patients and methods Fifty-three bone marrow biopsy samples with B-NHL were included in the study for the expression of t(11;14) BCL1/IgH in various B-cell lymphomas by FISH and MDR1/P-gp by immunohistochemistry and its relation to the outcome and chemotherapy resistance. Results In this study t(11;14) detected by FISH was positive in 64.2%. It was positive in (66.7%) of mantle cell lymphoma, in 64.3% of small cell lymphoma, and in 60% of large cell lymphoma. The detection frequency of MDR1/P-gp by immunohistochemistry in mantle cell lymphoma, small cell lymphoma and large cell lymphoma, is 80, 82, and 80%, respectively. Conclusion In this study, the frequency of t(11;14) detection by FISH and MDR1/P-gp expression by immunohistochemistry in Egyptian patients were consistent with other studies and the coexistence of MDR1/P-gp and t(11;14) in B-NHL is important before treatment to allow the design of novel drug regimens.


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