The Egyptian Journal of Haematology

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 40  |  Issue : 2  |  Page : 66--73

Frequency of the ALK gene and its prognostic value in neuroblastoma by FISH


Muhammad Raa'fat Khalaf, Eman Mosaad Zaki, Abeer Moustafa Darwish, Mohamed Galal Mostafa El-Naggar 
 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Correspondence Address:
Mohamed Galal Mostafa El-Naggar
Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut 71515
Egypt

Introduction The anaplastic lymphoma kinase (ALK) gene has been identified as a major neuroblastoma (NB) predisposition gene. Furthermore, there are controversies on the correlation between ALK gene aberration and clinical outcome in neuroblastoma (NBL). Materials and methods We evaluated N-MYC/ALK gene copy number by fluorescence in situ hybridization and analyzed 32 bone marrow samples infiltrated by NB and analyzed their association with the clinical outcome of the patients. Results Although an increase in ALK gene aberration is a recurrent genetic abnormality of NB (50%, 16/32), ALK amplification was only present in one NB (3.2%, 1/32). In addition, ALK positivity also significantly correlates with N-MYC gene copy number (P < 0.000). Kaplan-Meier survival analysis indicated that the N-MYC/ALK aberration is correlated with decreased overall survival (OS) in NB. A better prognosis was observed in patients who were negative for N-MYC/ALK normal compared with those who were positive for N-MYC/ALK aberration tumors. Furthermore, ALK aberrations were significantly correlated with inferior survival in NB (P < 0.000). Conclusion ALK aberrations in NB were correlated with advanced tumor types and an increase in both N-MYC/ALK gene aberrations. ALK aberrations predict an inferior prognosis, which can be used as a prognostic factor in NB in clinical practice.


How to cite this article:
Khalaf MR, Zaki EM, Darwish AM, El-Naggar MM. Frequency of the ALK gene and its prognostic value in neuroblastoma by FISH.Egypt J Haematol 2015;40:66-73


How to cite this URL:
Khalaf MR, Zaki EM, Darwish AM, El-Naggar MM. Frequency of the ALK gene and its prognostic value in neuroblastoma by FISH. Egypt J Haematol [serial online] 2015 [cited 2020 Jan 18 ];40:66-73
Available from: http://www.ehj.eg.net/article.asp?issn=1110-1067;year=2015;volume=40;issue=2;spage=66;epage=73;aulast=Khalaf;type=0