The Egyptian Journal of Haematology

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 42  |  Issue : 3  |  Page : 123--127

Oxidative stress in pediatric patients with β thalassemia major


Asmaa Nafady1, Sanaa Shaker Ali2, Hosny Mohammed Ahmed El Masry3, Khaled Abdel Baseer4, Heba M Qubaisy4, Samar Gallab Mahmoud4, Hanaa A Nafady-Hego5 
1 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt
2 Clinical and Chemical Pathology, Qena Faculty of Medicine, South Valley University, Qena, Egypt
3 Department of Pediatric, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
4 Department of Pediatric, Faculty of Medicine, Assiut University, Assiut, Egypt
5 Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Correspondence Address:
Asmaa Nafady
Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut
Egypt

Background β-thalassemia major (β-TM) is a common inherited hemolytic type of anemia. Repeated blood transfusions predispose β-TM patients toward peroxidative tissue injury because of secondary iron overload. Objectives This study aimed to evaluate the effects of iron overload on antioxidant enzymes and liver cell damage in β-TM patients undergoing regular blood transfusions. Patients and methods This prospective case–control cohort study included 30 pediatric patients with a confirmed diagnosis of β-TM on regular blood transfusions and 20 age-matched and sex-matched healthy children attending the Qena University Hospital, Pediatric Clinic. Blood samples were withdrawn from each patient to measure serum levels of ferritin, glutathione peroxidase (GPX), and superoxide dismutase (SOD). Results Total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), and ferritin levels were significantly higher in the β-TM group (P<0.001, 0.001, 0.001, and 0.001, respectively), whereas GPX and SOD were significantly lower in the β-TM group (P<0.001 and 0.001). The correlation between serum ferritin level and age, bilirubin, AST, and ALT in patients group showed that, the correlation between serum ferritin level and age was (0.745) while P-value was <0.001, the correlation between serum ferritin level and bilirubin level was (0.665) while P-value was <0.001, the correlation between serum ferritin level and (AST) level was (0.727) while P-value was <0.001 and the correlation between serum ferritin level and (ALT) level was (0.737) while P-value was <0.001. The correlation between SOD and age, ferritin, bilirubin, AST, and ALT in patients group in patients group showed that, the correlation between (SOD) and age was (−0.454) while P-value was 0.012, the correlation between (SOD) and ferritin level was (−0.664) while P-value was <0.001, the correlation between (SOD) and bilirubin level was (−0.535) while P-value was 0.002, the correlation between (SOD) and (AST) level was (−0.567) while P-value was <0.001 and the correlation between (SOD) and (ALT) level was (−0.558) while P-value was <0.001. Conclusion Impaired levels of antioxidant enzymes SOD and GPX in patients with β-TM on repeated transfusion, in addition to excessive free iron concentration, iron overload may attribute to oxidative damage in these patients. Antioxidant systems that compensate for reduced lipid peroxidation to lower tissue damage are needed.


How to cite this article:
Nafady A, Ali SS, El Masry HA, Baseer KA, Qubaisy HM, Mahmoud SG, Nafady-Hego HA. Oxidative stress in pediatric patients with β thalassemia major.Egypt J Haematol 2017;42:123-127


How to cite this URL:
Nafady A, Ali SS, El Masry HA, Baseer KA, Qubaisy HM, Mahmoud SG, Nafady-Hego HA. Oxidative stress in pediatric patients with β thalassemia major. Egypt J Haematol [serial online] 2017 [cited 2020 Sep 20 ];42:123-127
Available from: http://www.ehj.eg.net/article.asp?issn=1110-1067;year=2017;volume=42;issue=3;spage=123;epage=127;aulast=Nafady;type=0