The Egyptian Journal of Haematology

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 43  |  Issue : 2  |  Page : 45--48

Screening of single nucleotide polymorphism in CD95 (APO-1/FAS) promoter region (G-1377A) in children with acute leukemia


Dilara F Akin1, Didem T Özkan2, Emin Kürekçi3, Nejat Akar4 
1 Department of Medical Biology, Faculty of Medicine, Niğde Ömer Halisdemir University, NNiğdee, Turkey
2 Vocational School of Health Service, Okan University, İstanbul, Turkey
3 LÖSANTE Children’s and Adult Hospital, Ankara, Turkey
4 TOBB-ETU Hospital, Ankara, Turkey

Correspondence Address:
Dilara F Akin
Department of Medical Biology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde
Turkey

Background CD95 is a cell surface receptor involved in apoptotic signal transmission. Deregulation of this pathway results in downregulation of apoptosis and subsequent persistence of a malignant clone. A single nucleotide polymorphism resulting in guanine-to-adenine (G>A) transition in the CD95 promoter region (position −1377) is thought to reduce stimulatory protein 1 transcription factor binding and decrease CD95 expression. The purpose of this study is to examine a genetic polymorphism in the core promoter region of CD95 and to evaluate association between its frequency and clinical findings. Patients and methods G-1377A in the CD95 promoter region was genotyped by polymerase chain reaction and restriction endonuclease analysis finally were sequenced by Sanger Sequecing. Results Among 146 patients, CD95 G-1377A (rs2234767) single nucleotide polymorphism carriers frequencies have been identified as 25% (n=37) GA and AA 4% (n=6). This polymorphism of the distribution of the CD95 gene in children with acute leukemia will be a guide for future studies. Conclusion This polymorphism of the distribution of the CD95 gene in children with acute leukemia will be a guide for future studies.


How to cite this article:
Akin DF, Özkan DT, Kürekçi E, Akar N. Screening of single nucleotide polymorphism in CD95 (APO-1/FAS) promoter region (G-1377A) in children with acute leukemia.Egypt J Haematol 2018;43:45-48


How to cite this URL:
Akin DF, Özkan DT, Kürekçi E, Akar N. Screening of single nucleotide polymorphism in CD95 (APO-1/FAS) promoter region (G-1377A) in children with acute leukemia. Egypt J Haematol [serial online] 2018 [cited 2018 Dec 11 ];43:45-48
Available from: http://www.ehj.eg.net/article.asp?issn=1110-1067;year=2018;volume=43;issue=2;spage=45;epage=48;aulast=Akin;type=0