The Egyptian Journal of Haematology

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 44  |  Issue : 2  |  Page : 149--156

Correlation of B cell activating factor, B cell maturation antigen, and other cytokines profile with inhibitor status in hemophilia A


Sanya Arshad1, Vandana Tiwari2, Nuzhat Husain1, Azfar Neyaz1, Namrata P Awasthi1 
1 Department of Pathology, Dr Ram Manohar Lohia Institute of Medical Sciences, Gomti Nagar, Lucknow, Uttar Pradesh, India
2 Department of Biochemistry, Dr Ram Manohar Lohia Institute of Medical Sciences, Gomti Nagar, Lucknow, Uttar Pradesh, India

Correspondence Address:
Nuzhat Husain
Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Gomti Nagar, Lucknow 226010, Uttar Pradesh
India

Background The role of cytokines in inhibitor-positive versus inhibitor-negative patients with hemophilia A (PWHA) is controversial. Raised levels of B cell activating factor (BAFF) and B cell maturation antigen (BCMA) have been recently reported and have been linked to presence of inhibitors. Aim To directly quantify serum BAFF and BCMA along with interleukin (IL)-10, IL-5, and tumor necrosis factor-α (TNF-α) in PWHA following an on-demand schedule for factor VIII replacement, and to correlate these with demographic, clinicopathological, and treatment parameters. Patients and methods The study group included 35 inhibitor-positive, 131 inhibitor-negative PWHA, and 20 healthy controls. Serum levels of BAFF, BCMA, IL-10, IL-5, and TNF-α were determined by enzyme-linked immunosorbent assay. Results The mean serum levels in inhibitor positive, inhibitor negative, and healthy controls for BAFF was 0.346, 0.218, and 0.118 pg/ml (P=0.280), for BCMA was 0.266, 0.176, and 0.187 pg/ml (P=0.298), for IL-10 was 13.71, 5.72, and 2.45 pg/ml (P≤0.001), for IL-5 was 2.90, 2.74, and 2.50 pg/ml (P=0.205) and for TNF-α was 5.20, 6.78, and 4.0 pg/ml (P=0.558), respectively. Six (30%) inhibitor-positive PWHA had elevated BCMA levels while none of the inhibitor-negative PWHA showed increased values. Conclusion The current study has, for the first time, analyzed BAFF and BCMA in PWHA with inhibitor status in a clinical setting of on-demand therapy. As a preliminary conclusion we feel that, while cytokines play a role in pathogenesis they do not seem to trigger inhibitor development in PWHA.


How to cite this article:
Arshad S, Tiwari V, Husain N, Neyaz A, Awasthi NP. Correlation of B cell activating factor, B cell maturation antigen, and other cytokines profile with inhibitor status in hemophilia A.Egypt J Haematol 2019;44:149-156


How to cite this URL:
Arshad S, Tiwari V, Husain N, Neyaz A, Awasthi NP. Correlation of B cell activating factor, B cell maturation antigen, and other cytokines profile with inhibitor status in hemophilia A. Egypt J Haematol [serial online] 2019 [cited 2020 Sep 20 ];44:149-156
Available from: http://www.ehj.eg.net/article.asp?issn=1110-1067;year=2019;volume=44;issue=2;spage=149;epage=156;aulast=Arshad;type=0