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  Indian J Med Microbiol
 

Figure 1: Detection of the

Figure 1: Detection of the <iHFE</i H63D mutation by PCR-RFLP. Lane M: DNA marker (50 bp) (Fermentas, Germany). Lanes 1, 2, 3, 5, 6, 7, 8, 10, and 12 show a normal (wild) genotype with restriction fragments of 138, 99, and 57 bp. Lanes 4 and 9 show heterozygous genotypes with restriction fragments of 138, 99, and 57 bp in addition to an undigested band (237 bp). RFLP, restriction fragment length polymorphism.